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KMID : 0545119980080060575
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 6 p.575 ~ p.580
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Production of Cellulase by Trichoderma reesei Rut C30 in a Batch Fermenter
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Yu, Xiao Bin
Yun, Hyun Shik/Koo, Yoon Mo
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Abstract
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Cellulase production by batch culture using the Trichoderma reesei Rut C30 strain with various concentrations of Solka Floc with 1% wheat bran was studied in a 2.5 l fermenter. The cellulase activity increased with Solka Floc concentration up to 5%. When 5% Solka Floc and 1% wheat bran were contained in the medium, carboxymethyl cellulose (CMC) and filter paper (FP) activities were 232.4 U/㎖ and 21.25 U/㎖, respectively. The productivity was 143.6 FPU 1^-1h^-1 and the yield was 425 FPU/g. The colonial morphology of T. reesei Rut C30 grown on Avicel agar plates and the changes in mycelial morphology of T. reesei Rut C30 with culture time are also presented.
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